In vitro Diagnostics

Highly selective nucleic acid extraction. 

Chitosan, as a polycationic polymer, ensures strong and reversible electrostatic absorption of DNA. As well as repulsion of all positively charged molecules and irreversible absorption of (or lack of interaction with) all the usual PCR inhibitors. Usually, PCR inhibitors are found in animal and plant extracts and non-living environments:

PCR inhibitors, DNA absorption and release

Rapid and high-capacity binding.

Novochizol coating of magnetic beads generates a “peaks and troughs” texture, significantly increasing the surface area of nucleic acid absorption, with dynamic traps for both DNA and RNA:

Rapid and high capacity binding, regular chitosan coating versus Novochizol coating

Compatible with a wide variety of magnetic beads.

Both plain and silica-coated magnetic beads may be easily coated with Novochizol  yielding cleaner nucleic acids for more sensitive and more specific regular and real-time PCR assays.

Sample real time PCR and RT-PCR assays using Novobead in inhibitor-containing samples (tissue homogenates – trachea, lungs, liver, kidney – pooled from 20 chicken):

Typical real time RT-PCR Assay of Infectious Bronchitis Virus (IBV) (RNA marker). Nucleic acid isolation and PCR were carried out using RealBest kits ( https://vector-best.ru/en/) with either the supplied magnetic beads or Novobead (the nucleic acid extraction conditions were not optimized for Novobead):

Sample real time PCR and RT-PCR assays using Novobead in inhibitor-containing samples

Sample  real time PCR and RT-PCR assays using Novobead in inhibitor-containing samples*:

PCR and RT-PCR assays: Avian influenza A virus versus Chicken Anemia virus versus Infectious Bronchitis Virus

Modified Novobead can remove all nucleic acids from any sample.

Novobead with additional cross-linking agents enables quick and complete removal of all nucleic acids from cell lysates or other complex mixtures, as demonstrated by negative real-time PCR and RT-PCR results after 50 cycles.

DISCOVER HOW FAR YOU CAN PUSH THE BOUNDARIES OF YOUR PCR DIAGNOSTICS WITH NOVOBEAD